Genetic Relationship and Similarity of Some Chicken Strains
AbstractBlood samples were collected from the four chicken strain (twenty males and twenty females). (Ross, Indian River as meat type and, ISA Brown, Hy Line Brown as egg type chicken ) . Blood samples were collected in 3 ml tubes containing EDTA. Genomic DNA was extracted from 300 μl of blood. DNA purity and concentrations have been measured by Nano Drop® spectrophotometer. Random amplification of polymorphic DNA (RAPD-PCR) was done by using 35 primers from GenScript USA company. A total of 21 Primers gave results to find a complementary DNA Genomic sites. The PCR program included an initial denaturation step at 94 °C for 5 minutes followed by 40 cycles with 94 °C for 1 minutes for DNA denaturation, annealing as mentioned with each primer, extension at 72 °C for 1 minutes and final extension at 72 °C for 5 minute were carried out. The PCR products were tested with electrophoresis on 2 % agarose gels in 1x TBE buffer stained by Ethidium bromide. The amplified pattern was visualized on a UV trans and photographed .Statistical Analysis of RAPD bands were scored for their presence (1) or absence (0). The index of similarity between each two population’s genetic distances was calculated. Polymorphism of each primer was calculated. The highest number of bands was 134 bands among all groups used and which was created by the OPA-13 Primer, and the lowest number of bands was 5 bands, which was created by OPA-03 Primer. The total number of bands created by all the Primers was 1724 and the total number of polymorphic band created by all the Primers was 216. The Primer OPQ-O4 had the highest number of polymorphic bands being 18 bands. While the Primer OPA-15 possessed the lowest number of polymorphic band being 2 bands. The average number was 12.77 of polymorphic bands per primer. The highest percentage of the Polymorphisms observed in the primer OPA-19, was 29.09% when compared with other primers in this study, where the lowest percentage of Polymorphisms was of primer of OPA-15 being 4.55%. A high level of genetic similarity was also observed between the meat type chickens and it was 0.741 between Ross ♀ and Indian ♀. A low level of genetic similarity was also observed between the meat and eggs chickens being 0.587 between Ross ♀ and ISA ♀.
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