Susceptibility Pattern and Detection of Some Antibiotic Resistance Genes in Staphylococcus aureus

Zeerak F. Ahmed, Rastee Hassan Saeed1, Safa A. Naje

Abstract


In this study a total of fifty samples were collected from patients who were admitted to West Erbil Emergency, Emergency, and Rizgary teaching Hospitals during the period from 1 October 2015 to 20 February 2016. Samples collected from different clinical sources: 20 from burns, 10 from surgical wounds, 10 from dental carries, and 10 from urine samples. Isolates were identified using cultural, morphological, biochemical tests, and confirmed by VITEK2 compact system. Twenty isolates were identified as Staphylococcus aureus. Antibiotic Sensitivity test by disk diffusion method was done for all S. aureus isolates against 20 commonly used antibiotics and the resistance percentage was as the following: 100% for AMC, AP, AX, and PG, 90% for ME, 65% for CAZ, 65% for TM, 60% for T, 60% for KF ,50% for CRO, 45% for CTX, and S, 45% for L, 40% for RA, 40% for DA, 35% for CIP, 35% for C%15, for KF and 15% for GM, and 10% for E, while all isolates were sensitive to vancomycin. PCR technique was used for the detection of tetk, blaZ, and Fem(A), gene in S. aureus isolates and the results showed that 7 (35%),18(90%), and 11(55%) of isolates were tetk, blaZ, and Fem(A) positive respectively.

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References


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