Assessment of Azadirachta indica seed kernel extracts to restrict the rampancy of antinematicidal –resistant Haemonchus contortus in ovine
The current study was executed to evaluate the potency of crude aqueous methanol extract (CAME) of Azadirachta (A.) indica seed kernels in controlling the prevalence of oxfendazole (OXF), levamisole HCL (LEV) and ivermectin (IVM)-resistant Haemonchus (H.) contortus in sheep. Faecal egg count reduction test (FECRT), calculated by the RESO program, revealed an emergence of resistance among the parasite populations against the aforementioned antinematicidals particularly OXF [FECR% = -56 and lower confidence interval (LCI) = -311]. The recorded FECR% for LEV and IVM was 75 and 78 whilst the LCI for both of them was 38 and 42, respectively. Egg hatch assay (EHA) also exhibited a disastrous level of resistance in H. contortus toward OXF. Antinematicidal activity of A. indica seed kernels was assessed using FECRT, EHA and adult motility test (AMT). Mean egg per gram of faeces (EPG) recorded post-treatment of sheep at low (2g g ־k¹ BW) and high (4g kg ־¹ BW) doses of A. indica CAME was 539±367.11 SE and 147±58.45 SE, respectively, as compared to the control group (mean EPG= 987±364.26 SE). There was no significant difference statistically (P>0.05) in FECR% (45.62 vs 85.14) in sheep at low and high doses of the plant. The results of AMT revealed a dose and time dependent efficacy of CAME of the assessed plant to kill antinematicidal-resistant adult worms with calculated LC50 values of 52.20, 15.40, 3.26, 1.55 and 0.80 mg ml-1 after 2, 4, 6, 8 and 10 hours. Accordingly, high dose (4g kg ־¹ BW) of A. indica seed kernel extract could be used to treat sheep carrying antinematicidal- resistant populations of H. contortus.
Babar, W., 2005. Worm control practices and anthelmintic resistance nematodes of sheep and goats at livestock farm Jaba and its surroundings (NWFP). M.Sc Thesis, Dept. Vet. Parasitol. Univ. Agri., Faisalabad, Pakistan.
Bartley, D.J., Jackson, F., Jackson, E. and Sargison, N., 2004. Characterisation of two triple resistant field isolates of Teladorsagia from Scottish lowland sheep farms. Veterinary Parasitology, 123, 189-199.
Bowman, D.D., 2009. Georgi’s Parasitology for Veterinarians. 9th Ed., Saunders Elsevier, printed in China, pp. 161-162.
Bowman, D.D., Lynn R.C. and Eberhard, M.L., 2003. Georgi’s Parasitology for Veterinarian. 8th Ed., Saunders Book Co., Philadelphia, USA, pp. 422.
Butterworth, J.H. and Morgan, E.D., 1968. Isolation of a substance that suppresses feeding in locusts. Chemistry Community, 23-24.
Cala, A.C., Chagas, A.C.S., Oliveira, M.C.S., Matos, S.A.P., Borges, L.M.F., Sousa, L.A.D., Souza, F.A. and Oliveira, G.P., 2012. In vitro Anthelmintic effect of Melia azedarach L. and Trichilia claussenii C. against sheep gastrointestinal nematodes. Experimental Parasitology, 130, 98-102.
Coles, G.C., 2005. Anthelmintic resistance-looking to future: a UK perspective. Research in Veterinary Science, 78, 99-108.
Coles, G.C., Bauer, C., Borgsteede, F.H.M., Greets, S., Klei, T.R., Taylor, M.A. and Waller, P.J., 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, 44, 35-44.
Costa, C.T.C., Bevilaqua, C.M.L., Camurca-Vasconcelos, A.L.F., Maciel, M.V., Morais, S.M., Castro, C.M.S., Braga, R.R. and Oliveira, L.M.B., 2008. In vitro ovicidal and larvicidal activity of Azadirachta indica extracts on Haemonchus contortus. Small Ruminant Research, 74, 284-287.
Costa, C.T.C., Bevilaqua, C.M.L., Maciel, M.V., Camurca-Vasconcelos, A.L.F., Morais, S.M., Monteiro, M.V.B., Farias, V.M., Da Silva, M.V. and Souza, M.M.C., 2006. Anthelmintic activity of Azadirachta indica against sheep gastrointestinal nematodes. Veterinary Parasitology, 137, 306-310.
Croom, E.M., 1983. Documenting and evaluating herbal remedies. Economic Botany, 37, 13-27.
Dhawan, B.N. and Patnaik, G.K., 1993. Pharmacological studies for therapeutic potential. In: Randhawa, N.S.; Parmer, B.S. editors. Neem research and development. Society of Pesticide Science, New Delhi, India, pp: 242-249.
Farooq, S., Mushtaq, Z., Iqbal, Z. and Akhtar, S., 2012. Parasitic helminths of domesticated and wild ruminants in cholistan desert of Pakistan. International Journal of Agriculture and Biology, 14, 63–68.
Getachew, T., Dorchies, P. and Jacquiet, P., 2007. Trends and challenges in the effective and sustainable control of Haemonchus contortus infection in sheep. Addis Ababa Univ., Faculty of Veterinary Medicine, Debre Zeit, Ethiopia. Parasitology, 1, 3-14.
Ghisi, M., Kaminsky, R. and Master, P., 2007. Phenotyping and genotyping of Haemonchus contortus isolates reveals a new putative candidate mutation for benzimidazole resistance in nematodes. Veterinary Parasitology, 144, 313-320.
Gilani, A.H., Ghayur, M.N., Saify, Z.S., Ahmad, S.P. Choudary, M.I. and Khalid, A., 2004. Presence of cholinomimetic and acetylcholinesterase inhibitory constituents in betel nut. Life Science, 75, 2377-2389.
Gill, B.S., 1996. Anthelmintic resistance in India. Veterinary Parasitology, 63, 173-176.
Hamad, K.K., Iqbal, Z., Sindhu, Z.U.D. and Muhammad, G., 2013. Antinematicidal activity of Nicotiana tabacum L. leaf extracts to control benzimidazole-resistant Haemonchus contortus in sheep. Pakistan Veterinary Journal, 33, 85-90.
Hammond, J.A., Fielding, D. and Bishop, S.C., 1997. Prospect for plant anthelmintics in Tropical Veterinary Medicine. Veterinary Research Communications, 21, 213-228.
Hördegen, P., Hertzberg, H., Heilmann, J., Langhans, W. and Maurer, V., 2003. The anthelmintic efficacy of five plant products against gastrointestinal trichostrongylids in artificially infected lambs. Veterinary Parasitology, 117, 51-60.
Hubert, J. and Kerboeuf, D., 1992. A microlarval development assay for the detection of anthelmintic resistance in sheep nematodes. Veterinary Record, 130, 442-446.
Iqbal, Z., Lateef, M., Jabbar, A., Ghayur, M.N. and Gilani, A.H., 2006. In vitro and in vivo anthelmintic activity of Nicotiana tabacum L. leaves against gastrointestinal nematodes of sheep. Phytotherapy Research, 20, 46-48.
Jabbar, A., Iqbal, Z., Kerboeuf, D., Muhammad, G., Khan, M.N. and Afaq, M., 2006. Anthelmintic resistance: The state of play revisited. Life Science, 79, 2413-2431.
Kelly, J.D., Hall, C.A., Whitlock, H.V., Thompson, H.G., Campbell, N.J. and Martin, I.C.A., 1977. The effect of route of administration on the anthelmintic efficacy of benzimidazole anthelmintics in sheep infected with strains of Haemonchus contortus and Trichostrongylus colubriformis resistant or susceptible to thiabendazole. Research in Veterinary Science, 22, 161-168.
Macedo, I.T.F., Bevilaqua, C.M.L., de Oliveira, L.M.B., Camurça-Vasconcelos, A.L.F., Viera, L. da S., Oliveira, F.R., Queiroz-Junior, E.M., Tome, A. da R. and Nascimento, N.R.F., 2010. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Veterinary Parasitology, 173, 93-98.
MAFF, 1986. Manual of Veterinary Parasitological Laboratory Techniques. Tech. Bull., No.18. Ministry of Agriculture Fisheries and Food. Her Majesty’s Stationary Office, London, UK.
Martin, P.J., Anderson, N. and Jarrett, R.G., 1989. Detecting benzimidazole resistance with faecal egg count reduction tests and in vivo assays. Australian Veterinary Journal, 66, 236-240.
Neveu, C., Charvet C., Fauvin, A., Cortet, J., Castagnone-Sereno, P. and Cabaret, J., 2007. Identification of levamisole resistance markers in the parasitic nematode, Haemonchus contortus using a c-DNA-AFLP approach. Parasitology, 134, 1105-1110.
Saddiqi, H.A., 2005. Benzimidazole resistance and associated contributing factors in gastrointestinal nematodes of sheep and goats. M.Sc Thesis, Dept. Vet. Parasitol. Univ. Agri., Faisalabad, Pakistan.
Soulsby, E.J.L., 1982. Helminths, Arthropods and Protozoa of Domasticated Animals.7th Ed., Bailliere Tindall, London, UK, pp. 248-250, 766-770.
Stear, M.J., Doligalska, M. and Donskov-Schmelter, K., 2007. Alternatives to anthelmintics for the control of nematodes in livestock. Parasitology, 134, 139-151.
Urquhart, G.M., Armour, J., Duncan, J.L., Dunn, A.M. and Jennings, F.W., 2007. Veterinary Parasitology. 2nd Ed., Blackwell Sciences Ltd., Oxford, UK. pp. 307.
Waller, P.J., 2006. From discovery to development: Current industry prospectives for the development of novel methods of helminth control in livestock. Veterinary Parasitology, 139, 1-14.
Waller, P.J., Barnes, G., Thamsborg, S.M., Sukura, A., Richter, S.H., Ingebrigtsens, K. and Hoglung, J., 2001. Plants have deworming agents of livestock in the Nordic countries: Historical prospective, popular beliefs and prospectus for the future. Acta Veterinaria Scandinavica Journal, 42, 31-44.
Wanzala, W., Zessin, K.H., Kyule, N.M., Baumann, M.P.O., Mathias, E. and Hassanali, A., 2005. Ethnoveterinary medicine: a critical review of its evolution, perception, understanding and the way forward. Livestock Research Rural Development, 17, 11.
Wood, I.B., Amaral, N.K., Bairden, K., Duncan, J.L., Kassai, T.K., Malone, J.B., Pankavich, J.A., Reinecke, P.K., Slocombe, O., Taylor, S.M. and Vercruysse, J., 1995. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition of guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Veterinary Parasitology, 58, 181-183.
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