Antioxidant, and Antimicrobial Activities of Phenolic and Flavonoid Rich Medicinal Plants (Fritillaria zagrica and Tulipa kurdica) Bulbs Collected in Kurdistan Region of Iraq

  • Mukhlis Hamad Aali Department of Biology, College of Science, Salahaddin University-Erbil, Kurdistan Region, Iraq
  • Nadhum J. Ismaiel Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq
  • Fadzilah A. Abdul Majid Institute of Marine Biotechnology, University Malaysia Terengganu, 21300 Kuala Terengganu, Terengganu, Malaysia.
Keywords: Fritillaria zagrica, Tulipa kurdica, Antioxidant, Antimicrobial Activity, TPC, TFC.


Fritillaria zagrica and Tulipa kurdica had been used as traditional herbal remedies since antiquity to treat human diseases in the Kurdistan region of Iraq. This is the first report and conceive to analyze these two medicinal plants based. Methanol, 80% ethanol and aqueous extracts of two medicinal plants (Fritillaria zagrcia and Tulipa kurdica) were evaluated for their antibacterial activity and antifungal activities. We have quantified the total phenolic (TPC) and flavonoid (TFC) contents and their relation to antioxidants (ABTS) [2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate) and 2,2′-diphenyl-1 picryl hydrazyl (DPPH)scavenging free radicals in a dose dependent method. Results showed that F. zagrica and T. kurdica bulb extractions by using different solvents exhibited strong Antimicrobial activities against selected bacterial and fungal strains except C. guilliermondii ATCC 6260. Aqueous bulb extract of F. zagrica and T. kurdica showed better antibacterial activity compared to other solvent extracts. Methanol F. zagrica bulb extracts contain significantly higher phenolic content than T. kurdica. The TPC and TFC contents were varied between the plant bulb extracts and the DPPH and ABTS scavenging activities were exhibited to be significantly correlated with the amount of TPC and TFC.


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AKTER, M. S., AHMED, M. & EUN, J.-B. 2010. Solvent effects on antioxidant properties of persimmon (Diospyros kaki L. cv. Daebong) seeds. International Journal of Food Science & Technology, 45, 2258-2264.
AL-REFAI, F. 2006. Isolation and identification of fungi from cosmetic using some plant extracts as preservative agents. Ph. D. Thesis. College of Science. Mosul Univ. Iraq.
BABA, S. A. & MALIK, S. A. 2014. Evaluation of antioxidant and antibacterial activity of methanolic extracts of Gentiana kurroo royle. Saudi J Biol Sci, 21, 493-8.
BHAGAT, J., LOBO, R., PARMAR, V. & BALLAL, M. 2011. In vitro Free Radical Scavenging Potential of Indian Habitant Anisochilus carnosus (LF) Wall. Chinese journal of natural medicines, 9, 456-460.
BHANDARI, J., MUHAMMAD, B., THAPA, P. & SHRESTHA, B. G. 2017. Study of phytochemical, anti-microbial, anti-oxidant, and anti-cancer properties of Allium wallichii. BMC Complement Altern Med, 17, 102.
BRAND-WILLIAMS, W., CUVELIER, M.-E. & BERSET, C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28, 25-30.
BRYAN, J. E. 2002. Bulbs, Portland, Oregon, U.S.A., Timber Press.
BRYAN, J. E. 2005. Pocket Guide to Bulbs ( Pocket Guides).pdf>, Portland, Oregon, U.S.A., Timber Press.
CHEN, C., LIU, C.-H., CAI, J., ZHANG, W., QI, W.-L., WANG, Z., LIU, Z.-B. & YANG, Y. 2018. Broad-spectrum antimicrobial activity, chemical composition and mechanism of action of garlic ( Allium sativum ) extracts. Food Control, 86, 117-125.
CHRISTENHUSZ, M. J. M., GOVAERTS, R., DAVID, J. C., HALL, T., BORLAND, K., ROBERTS, P. S., TUOMISTO, A., BUERKI, S., CHASE, M. W. & FAY, M. F. 2013. Tiptoe through the tulips - cultural history, molecular phylogenetics and classification ofTulipa(Liliaceae). Botanical Journal of the Linnean Society, 172, 280-328.
DZOYEM, J., PIEME, C. & PENLAP, V. 2010. In vitro antibacterial activity and acute toxicity studies of aqueous-methanol extract of Sida rhombifolia Linn.(Malvaceae). BMC complementary and alternative medicine, 10, 40.
GLISZCZYŃSKA-ŚWIGŁO, A. 2006. Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays. Food chemistry, 96, 131-136.
GLISZCZYNSKASWIGLO, A. 2006. Antioxidant activity of water soluble vitamins in the TEAC (trolox equivalent antioxidant capacity) and the FRAP (ferric reducing antioxidant power) assays. Food Chemistry, 96, 131-136.
HAO, D.-C., GU, X.-J., XIAO, P.-G. & PENG, Y. 2013. Phytochemical and biological research of Fritillaria Medicine Resources. Chinese Journal of Natural Medicines, 11, 330-344.
HAO, D. C., GU, X.-J. & XIAO, P. G. 2015. Phytochemical and biological research of Fritillaria medicinal resources. Medicinal Plants.
HATAMI, T., EMAMI, S. A., MIRAGHAEE, S. S. & MOJARRAB, M. 2014. Total phenolic contents and antioxidant activities of different extracts and fractions from the aerial parts of Artemisia biennis Willd. Iranian journal of pharmaceutical research: IJPR, 13, 551.
HOWELL, A. B. 2007. Bioactive compounds in cranberries and their role in prevention of urinary tract infections. Mol Nutr Food Res, 51, 732-7.
HUYUT, Z., BEYDEMIR, S. & GULCIN, I. 2017. Antioxidant and Antiradical Properties of Selected Flavonoids and Phenolic Compounds. Biochem Res Int, 2017, 7616791.
IQBAL, S., BHANGER, M., AKHTAR, M., ANWAR, F., AHMED, K. R. & ANWER, T. 2006. Antioxidant properties of methanolic extracts from leaves of Rhazya stricta. Journal of medicinal food, 9, 270-275.
ISMAIL, H. F., HASHIM, Z., SOON, W. T., RAHMAN, N. S. A., ZAINUDIN, A. N. & MAJID, F. A. A. 2017. Comparative study of herbal plants on the phenolic and flavonoid content, antioxidant activities and toxicity on cells and zebrafish embryo. J Tradit Complement Med, 7, 452-465.
JOHNSON, D. B., SHRINGI, B., PATIDAR, D. K., CHALICHEM, N. S. S. & JAVVADI, A. K. 2011. Screening of antimicrobial activity of alcoholic & aqueous extract of some indigenous plants. Indo-Global Journal of Pharmaceutical Sciences, 1, 186-193.
KALLEL, F., DRISS, D., CHAARI, F., BELGHITH, L., BOUAZIZ, F., GHORBEL, R. & CHAABOUNI, S. E. 2014. Garlic (Allium sativum L.) husk waste as a potential source of phenolic compounds: Influence of extracting solvents on its antimicrobial and antioxidant properties. Industrial Crops and Products, 62, 34-41.
KAMEL, F. H., ISMAEL, H. M. & MOHAMMADAMIN, S. A. 2014. Microbial deterioration of historical textiles and approaches for their control. Online Int Interdiscip Res J, 4, 10-17.
KHAN, S. U., ANJUM, S. I., ANSARI, M. J., KHAN, M. H. U., KAMAL, S., RAHMAN, K., SHOAIB, M., MAN, S., KHAN, A. J., KHAN, S. U. & KHAN, D. 2018. Antimicrobial potentials of medicinal plant’s extract and their derived silver nanoparticles: A focus on honey bee pathogen. Saudi Journal of Biological Sciences.
KIANI, M., SEFIDKON, F., BABAEI, A. & NAGHAVI, M. R. 2015. Phytochemical profiling of medicinal isosteroidal alkaloids of Iranian Fritillaria spp. (Liliaceae). Industrial Crops and Products, 70, 451-458.
KIM, K. H., LEE, K. W., KIM, D. Y., PARK, H. H., KWON, I. B. & LEE, H. J. 2004. Extraction and fractionation of glucosyltransferase inhibitors from cacao bean husk. Process Biochemistry, 39, 2043-2046.
KUMAR, J., DHAR, P., TAYADE, A. B., GUPTA, D., CHAURASIA, O. P., UPRETI, D. K., ARORA, R. & SRIVASTAVA, R. B. 2014. Antioxidant capacities, phenolic profile and cytotoxic effects of saxicolous lichens from trans-Himalayan cold desert of Ladakh. PloS one, 9, e98696.
LI, H. J., JIANG, Y. & LI, P. 2006. Chemistry, bioactivity and geographical diversity of steroidal alkaloids from the Liliaceae family. Nat Prod Rep, 23, 735-52.
LI, S., LIU, J., GONG, X., YANG, X., ZHU, Y. & CHENG, Z. 2013. Characterizing the major morphological traits and chemical compositions in the bulbs of widely cultivated Fritillaria species in China. Biochemical Systematics and Ecology, 46, 130-136.
LIANGLI, L. Y. 2008. Wheat antioxidants, New Jersey, U.S.A, John Wiley & Sons.
LIM, T. K. 2014. Edible Medicinal and Non-Medicinal Plants, Dordrecht Heidelberg New York London, Springer.
LIN, J.-Y. & TANG, C.-Y. 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food chemistry, 101, 140-147.
LIU, C., CHANG, J., ZHANG, L., ZHANG, J. & LI, S. 2012. Purification and antioxidant activity of a polysaccharide from bulbs of Fritillaria ussuriensis Maxim. Int J Biol Macromol, 50, 1075-80.
MARVIBAIGI, M., AMINI, N., SUPRIYANTO, E., ABDUL MAJID, F. A., KUMAR JAGANATHAN, S., JAMIL, S., HAMZEHALIPOUR ALMAKI, J. & NASIRI, R. 2016. Antioxidant Activity and ROS-Dependent Apoptotic Effect of Scurrula ferruginea (Jack) Danser Methanol Extract in Human Breast Cancer Cell MDA-MB-231. PLoS One, 11, e0158942.
MARVIBAIGI, M., AMINI, N., SUPRIYANTO, E., JAMIL, S., ABDUL MAJID, F. & KHANGHOLI, S. 2014. Total phenolic content, antioxidant and antibacterial properties of Scurrula ferruginea extracts. J Teknol, 5, 65-72.
MATSUO, Y., SHINODA, D., NAKAMARU, A. & MIMAKI, Y. 2013. Steroidal glycosides from the bulbs of Fritillaria meleagris and their cytotoxic activities. Steroids, 78, 670-82.
MAZHAR, A. 2014. In Vitro And In Vivo Toxicological Study Of Selective Medicinal Plants. University Of Agriculture, Faisalabad.
MOON, J.-W., CHEN-ZHI, C.-Z., SONG, W.-S. & BAEK, S.-H. 2011. Study on Antimicrobial Activity of Extracts from Fritillaria unibracteata Hsiao et KC Hsia and F. ussuriensis Maxim. Korean Journal of Plant Resources, 24, 719-723.
OTHMAN, A., MUKHTAR, N. J., ISMAIL, N. S. & CHANG, S. K. 2014. Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. International Food Research Journal, 21.
RE, R., PELLEGRINI, N., PROTEGGENTE, A., PANNALA, A., YANG, M. & RICE-EVANS, C. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine, 26, 1231-1237.
RUKSANS, J. 2007. Buried treasures: finding and growing the world's choicest bulbs. Portland, Oregon: Timber Press 384p., 76p. of col. plates. ISBN, 1241395189.
SANIEWSKI, M. & HORBOWICZ, M. Changes in endogenous flavonoids level during cold storage of tulip bulbs. VIII International Symposium on Postharvest Physiology of Ornamental Plants 669, 2003. 245-252.
SHARMA, K., MAHATO, N. & LEE, Y. R. 2018. Systematic study on active compounds as antibacterial and antibiofilm agent in aging onions. J Food Drug Anal, 26, 518-528.
SHIGETOMI, K., OLESEN, S. H., YANG, Y., MITSUHASHI, S., SCHONBRUNN, E. & UBUKATA, M. 2013. MurA as a primary target of tulipalin B and 6-tuliposide B. Biosci Biotechnol Biochem, 77, 2517-9.
SHIGETOMI, K., OMOTO, S., KATO, Y. & UBUKATA, M. 2011. Asymmetric total synthesis of 6-tuliposide B and its biological activities against tulip pathogenic fungi. Bioscience, biotechnology, and biochemistry, 75, 718-722.
SHIGETOMI, K., SHOJI, K., MITSUHASHI, S. & UBUKATA, M. 2010. The antibacterial properties of 6-tuliposide B. Synthesis of 6-tuliposide B analogues and structure-activity relationship. Phytochemistry, 71, 312-24.
SOBIA, N. 2011. Bioactivities And Phyto Chemical Analysis Of Four Plants Of Medicinal Importance. Quaid-i-Azam University, Islamabad.
SOMCHIT, M., REEZAL, I., NUR, I. E. & MUTALIB, A. 2003. In vitro antimicrobial activity of ethanol and water extracts of Cassia alata. Journal of Ethnopharmacology, 84, 1-4.
TEKŞEN, M. & AYTAÇ, Z. 2011. The revision of the genus Fritillaria L.(Liliaceae) in the Mediterranean region (Turkey). Turkish Journal of Botany, 35, 447-478.
TURKMEN, N., SARI, F. & VELIOGLU, Y. S. 2006. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods. Food chemistry, 99, 835-841.
WEATHERS, J. 1911. The bulb book; or, Bulbous and tuberous plants for the open air, stove, and greenhouse, containing particulars as to descriptions, culture, propagation, etc., of plants from all parts of the world having bulbs, corms, tubers, or rhizomes (orchids excluded), London :, J. Murray.
ZONNEVELD, B. J. M. 2009. The systematic value of nuclear genome size for “all” species of Tulipa L. (Liliaceae). Plant Systematics and Evolution, 281, 217-245.
How to Cite
Aali, M., Ismaiel, N. and Abdul Majid, F. (2018) “Antioxidant, and Antimicrobial Activities of Phenolic and Flavonoid Rich Medicinal Plants (Fritillaria zagrica and Tulipa kurdica) Bulbs Collected in Kurdistan Region of Iraq”, Zanco Journal of Pure and Applied Sciences, 30(5), pp. 1-16. doi: 10.21271/ZJPAS.30.5.1.