Molecular Identification of Acinetobacter baumanii and Acinetobacter genomic species 13TU Using PCR

  • Salah Tofik Jalal Balaky Department of Medical Microbiology, College of Health Science, Hawler Medical University, Erbil, Kurdistan Region, Iraq.
  • Haval Abdulkhalik Department of Medical Microbiology, College of Health Science, Hawler Medical University, Erbil, Kurdistan Region, Iraq.
  • Bashdar Mahmud Hussen Medical Research Center, Hawler Medical University, Erbil, Kurdistan Region, Iraq
  • Hozan Hassan Department of Medical Microbiology, College of Health Science, Hawler Medical University, Erbil, Kurdistan Region, Iraq.
  • Ahang Hasan Mawlood Department of Medical Microbiology, College of Health Science, Hawler Medical University, Erbil, Kurdistan Region, Iraq.
Keywords: Acinetobacter baumannii; genomic 13TU, gyrB gene, PCR, Resistance.

Abstract

Acinetobacter baumannii is well known to be multi-drug resistant and associated with many infectins. Manual and automated identification systems, can not differentiate between both species A. baumannii and Acinetobacter genomic species 13TU. The reason is the differences in nucleotide sequences in gyrB of both species, therefore, specific genotypic test required for differentiating them. The aim of this study is to differentiate between two species of Acinetobacter based on gyrB gene nucleotide sequence differences and their antibiotic resistance profile in Erbil city. Six hundred thirty two clinical specimens were collected during the period of March to August 2016. Colony, cultural morphology and VITEK2 system were used, in which 105 of them were A. baumannii. PCR was then used to differentiate between A. baumannii and Acinetobacter genomic species 13TU. Out of 632 clinical specimens, 105 were phenotypically identified as Acinetobacter baumannii using VITEK2 system. PCR results showed that Acinetobacter genomic species 13TU gave an amplicon of 294 bp as one band, but A. baumannii gave a second amplicon of 490 bp. The PCR method correctly identified 43 A. baumannii and 62 Acinetobacter genomic 13TU. Carbapenem resistance was observed 60% (n=40), infection among male 69.53% (n=73) was higher than female. PCR detection of Acinetobacter species showed high sensitivity and accuracy as compared to other phynotypic methods. To the best of our knowledge this is the first study performed to differentiate two important species of Acinetobacter in Kurdistan region.

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Published
2019-02-27
How to Cite
Balaky, S., H. Abdulkhalik, B. Hussen, H. Hassan, and A. Mawlood. “Molecular Identification of Acinetobacter Baumanii and Acinetobacter Genomic Species 13TU Using PCR”. ZANCO Journal of Pure and Applied Sciences, Vol. 31, no. 1, Feb. 2019, pp. 17-22, doi:10.21271/ZJPAS.31.1.3.