Cytogenetic analysis of human lymphocytes exposed to various concentrations of aqueous and methanolic extracts of Brassica oleracea var. italic (Broccoli)

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Twana Ahmed Mustafa
Hazha jawdat Jafar

Keywords

Cytogenetic effect / Human Lymphocyte / Brassica oleracea

Abstract

         The chromosomal aberration, Mitotic index (MI) were employed to investigate the in vitro effect of broccoli (Brassica oleracea) on human chromosomes. In vitro incubation for 48 hrs of normal human lymphocytes were studied with various concentrations (6.4, 3.2, 1.6, 0.8, 0.4 and 0.2 mg/ml) of Aqueous and Methanolic extract of broccoli. Results indicated a positive relationship between Mitotic index (MI) values and the methanolic extract at concentrations (0.19±0.012, 0.29±0.021, 0.26±0.003, 0.3±0.006, 0.32±0.011 and 0.34±0.008) respectively, and (0.12±0.006, 0.15±0.002, 0.19±0.011, 0.21±0.015, 0.22±0.021, 0.30±0.003) respectively, for aqueous extract, a significant differences noted (P≥0.01) among effects of different concentrations. MI value of positive control treatment cyclophosphamide (CP) (0.13±0.003) as compared with a negative control group (0.401±0.018). In addition the value of the damaged cells in negative control group was (0.06±0.01), whereas for positive control group was (43.65±0.003). The damaged cells for the treated group with extract revealed increasing in value in a concentration dependent manner from (4.99±0.08) to (26.53±0.004) and (5.75±0.08) to (23.43±0.023) for both aqueous and methanolic crude extract respectively as compared with the negative control (0.06±0.01). This effect maybe attributed to anti oxidative activity induced by Brassica oleracea

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