Molecular epidemiology of human Adenovirus among patients with Keratoconjunctivitis
Various Human Adenoviruses (HAdV) serotypes are reported to be accompanied with keratoconjunctivitis infections. Seventy-three conjunctival swabs were obtained from (39 male and 34 female) who presented to the ophthalmic unite in the hospital of Erbil province with clinical features of acute keratoconjunctivitis. Immunochromatography (IC) was performed 51(69.86%) samples were positive out of 73 eyes swab. In the present study, nested PCR was employed to amplify the partial hexon gene, and the sequence of the 37 isolates were specified and compared to sequences deposited in NCBI. The results illustrated that all isolates showed 96.7 to 100% nucleotide identity and all sequences were submitted to Genbank-NCBI which documented and received accessions number start from (MK615151 to MK615187) respectively. Phylogenetic analysis classified these isolates into different genotype-related groups A, B, D, E, and F and human adenovirus HAdV-D8 were the most frequent type. The results of nucleotide Blast of HAdV-D8 with the deposited sequence in NCBI were revealed that similar to genotype sequence documented in Germany in (99%).
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