Haemonchus contortus as a model in assessing activity of Citrullus colocynthis fruit extract to control benzimidazole-resistant parasitic nematodes

  • Chreska Nooraldin Ahmed Department of Biology, College of Science, Salahaddin University, Erbil- Kurdistan Region of Iraq
  • Kareem Khoshnow Hamad Department of Biology, College of Science, Salahaddin University, Erbil- Kurdistan Region of Iraq
  • Fikry Ali Qadir Department of Biology, College of Science, Salahaddin University, Erbil- Kurdistan Region of Iraq
Keywords: Haemonchus contortus, bitter apple, benzimidazole dewormer, resistance, alternative therapy

Abstract

The present field and laboratory experimental study were designed to appraise the efficiency of crude aqueous methanol extract (CAME) of Citrullus (C.) colocynthis (bitter apple) fruits in combating benzimidazole-resistant digestive canal nematodes. To meet the requirements of the trial, the highly pathogenic gastric roundworm of ovine, albendazole-resistant Haemonchus contortus was used as a paradigm. The faecal egg count reduction test (FECRT) and egg hatch assay (EHA) revealed prevalence of resistance to the abovementioned dewormer. In reference to the RESO Computer Program, the calculated FECR% was 70.77, whilst the lower confidence interval was 49.7. The LC50 value for albendazole, calculated via probit analysis, was 2.046 μg ml ־¹ after carrying out EHA.  Antinematicidal effectiveness of C. colocynthis CAME was assessed in vivo through administration of four doses (50, 100, 150, and 200 mg/kg-1 B.W) utilizing FECRT as an indication for their efficacies. There was a dose dependent antinematicidal potency of C. colocynthis CAME. The ovicidal activity of the plant extract was also trialed in vitro via conducting EHA. The estimated LC50 value was 0.3422 μg ml-1 after performing EHA, whilst the calculated mean FECR% for the highest dose of CAME was 95.57, which is deemed effective. Moreover, administration of C. colocynthis CAME at 200 mg/kg-1 B.W did not reveal untoward consequences in animals; accordingly, could be used as a substitute remedy in fighting antinematicidal- resistant populations of gastrointestinal nematodes.

References

Barnes, E.H., Dobson, R.J. and Barger, I.A., 1995. Worm control and anthelmintic resistance: adventures with a model. Parasitology Today, 11: 56-63.
Beech, R.N. and Silvestre, A., 2010. Mutations associated with anthelmintic drug resistance. Anti-infective agents in Medical Chemistry, 9: 105-112.
Bowman, D.D., Lynn, R.C. and Eberhard, M.L., 2003. Georgi’s Parasitology for Veterinarians. 8th Ed., Saunders Book Co., Philadelphia, pp. 422.
Coles, G.C., Bauer, C., Borgsteede, F.H.M., Greets, S., Klei, T.R., Taylor, M.A. and Waller, P.J., 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, 44: 35-44.
Coles, G.C., Papadopoulos, E. and Himonas, C.A., 1995. Tubulin, resistance and worms. Parasitology Today, 11: 183-185.
Gilani, A.H., Ghayur, M.N., Saify, Z.S., Ahmad, S.P., Choudary, M.I. and Khalid, A., 2004. Presence of cholinomimetic and acetylcholinesterase inhibitory constituents in betel nut. Life Science, 75: 2377-2389.
Gill, B.S., 1996. Anthelmintic resistance in India. Veterinary Parasitology, 63: 173-176.
Gilleard, J.S., 2013. Haemonchus contortus as a paradigm and model to study anthelmintic drug resistance. Parasitology, 140: 1506-1522.
Hamad, K.K., 2014. Combined strategies to control antinematicidal-resistant gastrointestinal nematodes in small ruminants on organized farms in Pakistan. Pakistan Journal of Agricultural Science, 51: 241-249.
Hamad, K.K., 2018. Assessment of Azadirachta indica seed kernel extracts to restrict the rampancy of antinematicidal-resistant Haemonchus contortus in ovine. ZANCO Journal for Pure and Applied Science, 30: 29-43.
Hamad, K.K., Ahmed, S.T., Ahmed, R.K. and Koyee, Q.M., 2018. Phytotherapeutics: As anticipating substitutes to synthetic drugs in combating antinematicidal-resistant gastrointestinal nematodes of small ruminants. ZANCO Journal for Pure and Applied Science, 30: 102-114.
Hamad, K.K., Iqbal, Z., Sindhu, Z.U.D. and Muhammad, G., 2013. Antinematicidal Activity of Nicotiana tabacum L. Leaf Extracts to Control Benzimidazole- Resistant Haemonchus contortus in Sheep. Pakistan Veterinary Journal, 33: 85-90.
Hamad, K.K., Iqbal, Z., Sindhu, Z.U.D., Abbas, R.Z., Khan, A., Muhammad, G. and Epperson, B., 2014. Combination of Nicotiana tabacum and Azadirachta indica: A Novel Substitute to Control Levamisole and Ivermectin-Resistant Haemonchus contortus in Ovine. Pakistan Veterinary Journal, 34: 24-29.
Hamad, K.K., Qadir, F.A. and Hamad, H.O., 2017. Control of antinematicidal-resistant gastrointestinal nematodes in tamed small ruminants: achievements, trends and prospectives. ZANCO Journal for Pure and Applied Science, 29: 62-77.
Harhay, M.O., Horton, J. and Olliare, P.L., 2010. Epidemiology and control of human gastrointestinal parasites in children. Expert Review of Anti-infective therapy, 8: 219-234.
Hussain, A.I., Rathore, H.A., Sattar, M.Z.A., Chatha, S.A.S., Sarker, S.D. and Gilani, A.H., 2014. Citrullus colocynthis (L.) Schard (bitter apple fruit): A review of its phytochemistry, pharmacology, traditional uses and nutritional potential. Journal of Ethnopharmacology, 155: 54-66.
Iqbal, Z., Sajid, M.S., Jabbar, A., Rao, Z.A. and Khan, M.N., 2006a. Techniques in Parasitology. Higher Education Commission, Islamabad, Pakistan, pp 198.
Iqbal, Z., Lateef, M., Jabbar, A., Ghayur, M.N. and Gilani, A.H., 2006b. In vivo anthelmintic activity of Butea monosperma against trichostrongylid nematodes in sheep. Fitoterapia, 77: 137-140.
Iqbal, Z., Nadeem, K.Q., Khan, M.N. and Waraich, F.N., 2001. Anthelmintic activity of Allium satvium, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. International Journal of Agriculture and Biology, 4: 451-453.
Jabbar, A., Iqbal, Z., Kerboeuf, D., Muhammad, G., Khan, M.N. and Afaq, M., 2006. Anthelmintic resistance: The state of play revisited. Life Science, 79: 2413-2431.
Kahn, C.M., 2005. The Merck Veterinary Manual, 9th Ed., Merck & Co., INC. Whitehouse Station, NJ., U.S.A., pp 2712.
Kaplan, R.M., 2004. Drug resistance in nematodes of veterinary importance: a status report. Trends in Parasitology, 20: 477-481.
Macedo, I.T.F., Bevilaqua, C.M.L., de Oliveira, L.M.B., Camurça-Vasconcelos, A.L.F., Viera, L. da S., Oliveira, F.R., Queiroz-Junior, E.M., Tome, A. da R. and Nascimento, N.R.F., 2010. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Veterinary Parasitology, 173: 93-98.
MAFF, 1986. Manual of Veterinary Parasitological Laboratory Techniques. Tech. Bull., No.18. Ministry of Agriculture Fisheries and Food. Her Majesty’s Stationary Office, London, UK.
Martin, P.J., Anderson, N. and Jarrett, R.G., 1989. Detecting benzimidazole resistance with faecal egg count reduction tests and in vivo assays. Australian Veterinary Journal, 66: 236-240.
Marzouk, B., Marzouk, Z., Décor, R., Edziri, H., Haloui, E., Fenina, N. and Aouni, M., 2009. Antibacterial and Anticandidal screening of Tunisian Citrullus colocynthis Schrad. from Medenine. Journal of Ethnopharmacology, 125: 344-349.
Rahuman, A.A., Venkatesan, P. and Gopalakrishnan, G., 2008. Mosquito larvicidal activity of oleic and linoleic acids isolated from Citrullus colocynthis (Linn.) Schard. Parasitology Research, 103: 1383-1390.
Rasool, K. and Jahanbakhsh, T., 2011. Anticandidal screening and antibacterial of Citrullus colocynthis in South East of Iran. Journal of Horticulture and Forestry.3: 382-398.
Saba, A.B. and Oridupa, A.O., 2010. Search for a novel antioxidant, anti-inflammatory/analgesic or anti-proliferative drug: Cucurbitacins hold the ace. Journal of Medicinal Plants Research, 4: 2821-2826.
Sindhu, Z.U.D., Iqbal, Z., Ahmad, A. and Abbas, R.Z., 2014. In vitro Ovicidal and Wormicidal Activity of Six Medicinal Plants against Haemonchus contortus. International Journal of Agriculture and Biology, 16: 1199-1203.
Soulsby, E.J.L., 1982. Helminths, Arthropods and Protozoa of Domasticated Animals.7th Ed., Bailliere Tindall, London, UK, pp. 248-250, 766-770.
Stear, M.J., Doligalska, M. and Donskov-Schmelter, K., 2007. Alternatives to anthelmintics for the control of nematodes in livestock. Parasitology, 134: 139-151.
Strain, S.A. and Stear, M.J., 2001. The influence of protein supplementation on the immune response to Haemonchus contortus. Veterinary Parasitology, 23: 523-531.
Vercruysse, J., Albonico, M., Behnke, J.M., Kotze, A.C., Prichard, R.K., McCarthy, J.S., Montresor, A. and Levecke, B., 2011. Is anthelmintic resistance a concern for the control of human soil-transmitted helminths? International Journal of Parasitology: Drugs and Drug Resistance, 1: 14-27.
Waller, P.J., 2006. From discovery to development: Current industry prospectives for the development of novel methods of helminth control in livestock. Veterinary Parasitology, 139: 1-14.
Waller, P.J., Dash, K.M., Barger, A., Le Jambre, L.F. and Plant, J., 1995. Anthelmintic resistance in nematode parasites of sheep: learning from the Australian experience. Veterinary Record, 136: 411-413.
Wood, I.B., Amaral, N.K., Bairden, K., Duncan, J.L., Kassai, T.K., Malone, J.B., Pankavich, J.A., Reinecke, P.K., Slocombe, O., Taylor, S.M. and Vercruysse, J., 1995. World Association of the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition of guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Veterinary Parasitology, 58: 181-183.
Published
2019-10-17
How to Cite
Nooraldin Ahmed, C., K. Hamad, and F. Ali Qadir. “Haemonchus Contortus As a Model in Assessing Activity of Citrullus Colocynthis Fruit Extract to Control Benzimidazole-Resistant Parasitic Nematodes”. ZANCO Journal of Pure and Applied Sciences, Vol. 31, no. 5, Oct. 2019, pp. 61-70, doi:10.21271/zjpas.31.5.8.
Section
Biology and Medical Researches