Polymorphism of miRNA and its Impact on IVF Failure
In vitro fertilization (IVF) failure is not only the cause of despair among couples and individuals undergoing the treatment, it´s also been contributing to the impediment of assistive reproductive technologies (ART) development, miRNAs have been linked to significant events in the reproduction course and the identification of miRNA polymorphisms may provide a good lead for the potential of diagnosis and treatment of unidentified IVF failure causes, the aim of the present study was to explore the association between the miRNA polymorphism (mir-125a T>Crs12975333) and IVF failure. Our case-control study consisted of 100 Kurdish women in total, 50 belong to the case group that underwent IVF failure and the other 50 belong to the control group who have had at least two successful pregnancies and no history of pregnancy loss, we used tetra amplification refractory mutation system to identify the polymorphisms within the groups, the TT genotype was found more frequently in IVF failure patients when compared to the healthy women (OR: 5.268, CI: 1.07-25.7, P=0.025) and T allele was more present in the case group (OR:1.9, CI:1.06-3.41, P=0.028). The difference in genotype and allele frequencies of mir-125a of the two groups may indicate that it has an effect on the target mRNAs and alter the implantation of embryo during IVF cycles.
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