Simultaneous Determination of Gallic Acid and Ascorbic Acid Using First Derivative Zero-Crossing Spectrophotometric Technique

  • Hemn A. Qader Pharmaceutical Chemistry Dept., College of Pharmacy, Hawler Medical University, Erbil, Kurdistan Region, IRAQ.
  • Nabil A. Fakhre Department of Chemistry, College of Education, Salahaddin University-Erbil, Kurdistan Region, IRAQ.
  • Sarmad B. Dikran Chemistry Department, College of Education-Ibn-Haitham, Baghdad, IRAQ
  • Hussein H. Hamad Soil Science, Directorate of Agriculture Research-Erbil, Kurdistan Region, IRAQ.
Keywords: Hibiscus sabdariffa L, Gallic acid, Ascorbic acid, Derivative spectrophotometry.


   A new spectrophotometric method was used for simultaneous determination of gallic acid and ascorbic acid depending on the first derivative zero-crossing technique. The first derivative spectra of these compounds permitted simultaneous determination of gallic acid and ascorbic acid over the concentration ranges of (1.0 – 30.0) μg/mL and (3.0 – 35.0) μg/mL with detection limits of 0.559 and 0.991 for gallic acid and ascorbic acid, respectively, by techniques of measuring the amplitude of peak-to-base line and zero-crossing at certain wavelengths. The proposed method showed reasonable precision and accuracy and have been applied to determine gallic acid and ascorbic acid in samples of Hibiscus sabdariffa L.


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How to Cite
A. Qader, H., A. Fakhre, N., B. Dikran, S. and H. Hamad, H. (2019) “Simultaneous Determination of Gallic Acid and Ascorbic Acid Using First Derivative Zero-Crossing Spectrophotometric Technique”, Zanco Journal of Pure and Applied Sciences, 31(s4), pp. 60-65. doi: 10.21271/zjpas.31.s4.11.