First Occurrence of Plesiomonas shigelloides Bacteria on/ in Carassius carassius and Silurus triostegus Fishes from Greater Zab River in Kurdistan Region, Iraq.
The present study shows occurrence of Plesiomonas shigelloides bacteria from Carassius carassius and Silurus triostegus that collected in Greater Zab River in Aski-Kalak in Erbil from October 2018 to November 2019. Swaps were taken from lesions on the skin, fins and internal organs of the 34 fishes (14 from C. carassius and 20 from S. triostegus) and inoculated in nutrient agar, blood agar and MacConkey agar. The agar plates were incubated at 37°C for 24-48 hours. Obtained 13 isolates, the bacterial isolates identified depending on some morphological, cultural, some biochemical test and further confirmed by Vitek 2 compound system. For characterization and identification the bacterial colonies were examined and then showed Gram negative reaction and motile. Biochemical tests were done by Vitek 2 compound system and result confirming. The study showed appearance of P. shigelloides on the skin lesions and intestine of the examined fish. The present existence of P. shigelloides bacteria regarded as first record for this bacterium in Iraq on and in fishes.
BEHERA,B. K.; BERA, A.K.; PARIA, P.; DAS, A.; PARIDA, P. K.; SUMAN KUMARI, BHOWMICK, S. & DAS, B. K. 2018. Identification and pathogenicity of Plesiomonas shigelloides in Silver Carp. Aquaculture, 493: 314-318.
BUTT, A. A.; FIGUEROA, J. & MARTIN, D. H. 1997. Ocular infection caused by three unusual marine organisms. Clinical Infectious Diseases, 24(4): 740.
BODHIDATTA, L.; MCDANIEL, P.; SORNSAKRIN, S.; SRIJAN, A.; SERICHANTALERGS, O. & MASON, C. J. 2010. Case-control study of diarrheal disease etiology in a remote rural area in Western Thailand. The American Journal of Tropical Medicine and Hygiene, 83(5): 1106–1109.
CHEN, X.; CHEN, Y.; YANG, Q.; KONG, H.; YU, F.; HAN, D.; ZHENG, S.; CUI, D. & LI, L. 2013. Plesiomonas shigelloides infection in Southeast China. PLoS One, 8 (11): 1-8. e77877
COAD, B. W. 2010. Freshwater fishes of Iraq. Pensoft Publisher, Sofia: 275 pp+ 16 plates.
FROESE, R. & PAULY, D. 2019. "FishBase", World web electronic publication. www.fishbase.org,version (2019).
GONZÁLEZ, C. J.; LOPEZ-DIAZ, T.; GARCIA-LOPEZ, M.; PRIETO, M. & OTERO, A. 1999. Bacterial microflora of wild brown trout (Salmo trutta), wild pike (Esox lucius), and aquacultured rainbow trout (Oncorhynchus mykiss). Journal of Food Protection, 62(11): 1270-1277.
HU, Q.; LIN, Q.; SHI, C.; FU, X.; LI, N.; LIU, L. & WU, S. 2014. Isolation and identification of a pathogenic Plesiomonas shigelloides from diseased grass carp. Wei Sheng Wu XueBao Acta Microbiologica Sinica, 54 (2): 229-235.
JANDA, J. M.; ABBOTT, S. L. & CHRISTOPHER J. MCIVER, C. J. (2016). Plesiomonas shigelloides Revisited. Clinical Microbiology Reviews, 29(2): 349-374. DOI: 10.1128/CMR.00103-15.
IBRAHIM, K. S. 2008. Pathological study of common carp (Cyprinus carpi) fish infected with Aeromonas hydrophila. M.Sc. Thesis, Coll. Vet. Med., Univ. Duhok: 89 pp.
KHATUN, H.; HOSSAIN, M. D.; JAHAN, S. N. & KHANOM, D. A. 2011. Bacterial infestation in different fish at rajshahi. Journal of Science Foundation, 9 (1&2): 77-84.
KLATTE, J. M.; DASTJERDI, M. H.; CLARK, K.; HARRISON, C. J. & GRIGORIAN, F. 2012. Hyperacute infectious keratitis with Plesiomonas shigelloides following traumatic lamellar corneal laceration. The Pediatric Infectious Disease Journal, 31: 1200-1201.
LIU, Z.; KE, X.; LU, M.; GAO, F.; CAO, J.; ZHU, H. & WANG, M. 2015. Identification and pathological observation of a pathogenic Plesiomonas shigelloides strain isolated from cultured tilapia (Oreochromis niloticus). . Wei Sheng Wu XueBao Acta Microbiologica Sinica, 55 (1): 96-106.
MOHAMMAD-ALI, N. R.; BALASEM, A. N. & MUSTAFA, S. R. 2000. Isolation of six bacterial species from Al-Zazfaraniya farm, South of Baghdad. veterinarian , 150-159.
MUSTAFA, K. K. 2015. Effects of Some Medical Plants on the Growth of Plesiomonas shigelloides Isolated from Different Water Sources. Duhok Journal, 1-9.
MUSTAFA, S. I. 2016. Disease agents of Cyprinus carpio from agriculture college fish farm in Erbil city- Kurdistan region. M.Sc. Thesis, Coll. Edu., Univ. Salahaddin: 89 pp.
NISHA, R.G.; RAJATHI, V.; MANIKANDAN, R. & PRABHU, N. M. 2014. Isolation of Plesiomonas shigelloides from infected Cichlid Fishes using 16S rRNA characterization and its control with probiotic Pseudomonas sp. Acta Scientiae Veterinariae, 42: 1195.
PINCUS, D. H. 2010. Microbial identification using the biomérieux vitek® 2 system. Hazelwood, MO, USA 32 pp.
PĘKALA-SAFIŃSKA, A. 2018. Contemporary threats of bacterial infections in freshwater fish. Journal of Veterinary Research, 62: 261-267.
SALEH, A. A. H. 1997. Evaluation of bacterial content in carp (Cyprinus carpio) and their immune responseagainst Aeromonas hydrophila M.Sc. Thesis, Coll. Sci., Univ. Basrah: 68pp.
THONGKAO, K. & SUDJAROEN, Y. 2017. Human pathogenic bacteria isolation from tilapia fishes (Oreochromis niloticus), a possible reservoir for zoonotic transmission. Annals of Tropical Medicine and Public Health, 10(6): 1563-1568.
OZDEMIR, O.; SARI, S.; TERZIOGLU, S. & ZENCIROGLU, A. 2010. Plesiomonas shigelloides sepsis and meningoencephalitis in a surviving neonate. Journal of Microbiology, Immunology and Infection, 43: 344-346.
WANG, X.; XU, L.; CAO, H.; WANG, J. & WANG, S. 2013. Identification and drug sensitivity of a Plesiomonas shigelloides isolated from diseased sturgeons. Wei Sheng Wu XueBao Acta Microbiologica Sinica, 53 (7): 723-729.
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