Rampancy of Antinematicidal Resistance Among Gastrointestinal Nematodes in Kurdish Goat Breeds

  • Kareem Khoshnow Hamad Department of Biology, College of Science, Salahaddin University- Erbil, Kurdistan Region of Iraq
  • Fikry Qadir Department of Biology, College of Science, Salahaddin University- Erbil, Kurdistan Region of Iraq
Keywords: Indigenous caprine, Alimentary tract nematodes, Synthetic dewormers, Antinematicidal resistance, Erbil province

Abstract

           The current study was designed to determine the emergence of resistance among gastrointestinal roundworms of native goats against renowned synthetic antinematicidals such as fenbendazole, avermectin and levamisole. To meet the survey requirements, six commercial goat farms, located in Sothern and Western Erbil province-Kurdistan region of Iraq, were chosen after performing qualitative parasitological assays. The study was executed from September to end of November, 2019. From each farm, 30 adult goats were haphazardly divided into two groups, a group (n=15) for treatment and other group (n=15) served as control. The faecal egg count reduction test (FECRT) and egg hatch assay (EHA) have disclosed emergence of resistance against fenbendazole. According to RESO Computer Program, the estimated FECR% in farms 1 and 2 were 70.72 and 79.55, whilst the lower confidence intervals 95% were 63 and 73.8 respectively. The LC50 value of fenbendazole after conducting EHA, calculated through probit analysis, was estimated to be 2.11 μg ml ־¹ (range 1.47-2.34). Regarding avermectin, the computed FECR% in farms 3 and 4 were 93.92 and 92.98, while the lower confidence intervals 95% were 92.91 and 90.7 respectively, which signified the presence of suspicion about prevalence of resistance against avermectin. For levamisole, the calculated FECR% in farms 5 and 6 were 95.99 and 96.38, whilst the lower confidence intervals 95% were 95.1 and 95.7 respectively. Consequently, the parasitic nematodes were susceptible to this synthetic chemotherapeutic in the region

References

Afaq, M., 2003. Parasite control practices and anthelmintic resistance against gastrointestinal nematodes of sheep. PhD thesis, Department of Veterinary Parasitology, University of Agriculture, Faisalabad, Pakistan.
Ahmed, C.N., Hamad, K.K. and Qadir, F.A., 2019. Haemonchus contortus as a model in assessing activity of Citrullus colocynthis fruit extract to control benzimidazole-resistant parasitic nematodes. ZANCO Journal of Pure and Applied Sciences, 31, 61-70.
Ahmed, I.A., Aziz, K.J. and Abdullah, S.O., 2015. Prevalence of Gastrointestinal Nematode Parasites in Sheep and Evaluation of Some Anthelmintic Resistance in Erbil Governorate. Basrah Journal of Veterinary Research, 14 (1).
Beech, R. N. and Silvestre, A., 2010. Mutations associated with anthelmintic drug resistance. Anti-infective agents in Medical Chemistry, 9: 105-112.
Besier, R.B., Kahn, L.P., Sargison, N.D. and Van Wyk, J.A., 2016. Chapter Four- The pathophysiology, ecology and epidemiology of Haemonchus contortus infection in small ruminants. Advanced Parasitology, 143, 93-95.
Blake, N. and Coles, G., 2007. Flock cull due to anthelmintic- resistance nematodes. Veterinary Record, 161, 36-36.
Bogan, J., Benoit, E. and Delatour, P., 1987. Pharmacokinetics of oxfendazole in goats: a comparison with sheep. Journal of Pharmacology and Therapy, 10, 305-309.
Chartier, C., Pors, I., Hubert, J., Rocheteau, D., Benoit, C. and Bernard, N., 1998. Prevalence of anthelmintic resistant nematodes in sheep and goats in Western France. Small Ruminant Research, 29, 33-41.
Coles, G.C., Bauer, C., Borgsteede, F.H.M., Greets, S., Klei, T.R., Taylor M.A. and Waller, P.J., 1992. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, 44, 35-44.
Coles, G.C., Papadopoulos, E. and Himonas, C.A., 1995. Tubulin, resistance and worms. Parasitology Today, 11, 183-185.
Falzon, L.C., Menzies, P.I., Shakya, K.P., Jones-Bitton, A., Vanleeuwen, J., Avula, J., Stewart, H., Jansen, J.T., Taylor, M.A., Learmount, J. and Peregrine, A.S., 2013. Anthelmintic resistance in sheep flocks in Ontario, Canada. Veterinary Parasitology, 193, 150-162.
Furgasa, W., Abunna, F., Yimer, L. and Haile, G., 2018. Review on anthelmintic resistance against gastrointestinal nematodes of small ruminants: Its status and perspective in Ethiopia. Journal of Veterinary Science and Animal Husbandry, 6, 407-418.
Geary, T.G., 2012. Are new anthelmintics needed to eliminate human helminthiasis? Current Opinion in Infectious Diseases, 25, 709-717.
Gill, B.S., 1996. Anthelmintic resistance in India. Veterinary Parasitology, 63, 173-176.
Gilleard, J.S., 2006. Understanding anthelmintic resistance: the need for genomics and genetics. International Journal for Parasitology, 36, 1227-1239.
Hamad, K.K., Ahmed, S.T., Ahmed, R.K. and Koyee, K.M., 2018. Phytotherapeutics: As anticipating substitutes to synthetic drugs in combating antinematicidal-resistant gastrointestinal nematodes of small ruminants. ZANCO Journal of Pure and Applied Sciences, 30, 102-114.
Hamad, K.K., 2012. Efficacy of some indigenous medicinal plants to control antinematicidal-resistant Haemonchus contortus in sheep. PhD thesis, Department of Parasitology, University of Agriculture, Faisalabad, Pakistan.
Hamad, K.K., Iqbal, Z., Sindhu, Z.U.D., Abbas, R.Z., Khan, M.N., Muhammad, G. and Epperson, B., 2014. Combination of Nicotiana tabacum and Azadirachta indica: A novel substitute to control levamisole and ivermectin-resistant Haemonchus contortus in ovine. Pakistan Veterinary Journal, 34, 24-29.
Hoekstra, R., Visser, A., Wiley, L.J., Weiss, A.S., Sangster, N.C. and Roos, M.H., 1997. Characterisation of an acetylcholine receptor gene of Haemonchus contortus in relation to levamisole resistance. Molecular and Biochemical Parasitology, 84, 179-187.
Iqbal, Z., Sajid, M.S., Jabbar, A., Rao, Z.A. and Khan, M.N., 2006. Techniques in Parasitology. Higher Education Commission, Islamabad, Pakistan, pp 198.
Jabbar, A., Iqbal, Z., Kerboeuf, D., Muhammad, G., Khan, M.N. and Afaq, M., 2006. Anthelmintic resistance: The state of play revisited. Life Science, 79, 2413-2431.
Kalkal, H., Vohra, S., Singh, S., Gupta, S., Magotra, A. and Bangar, Y.C., 2019. Detection of moderate to severe anthelmintic resistance against fenbendazole in sheep and goat breeding farms, Hisar. The Pharma Innovation Journal, 8, 434-436.
Kaplan, R.M. and Vidyashankar, A.N., 2012. An inconvenient truth: global warming and anthelmintic resistance. Veterinary Parasitology, 186, 70-78.
MAAF, 1986. Manual of Veterinary Parasitological Laboratory Techniques. Technical Bulletin No. 18. Ministry of Agriculture Fisheries and Food. Her Majesty's Stationary Office, London, UK.
Martin, P.J., Anderson, N. and Jarrett, R.G., 1989. Detecting benzimidazole resistance with faecal egg count reduction tests and in vivo assays. Australian Veterinary Journal, 66, 236-240.
Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D., 2007. Veterinary Medicine, a text book of the diseases of cattle, horses, sheep, pigs and goats. 10th Ed., Saunders Ltd., Philadelphia, USA, pp 2065.
Saeed, M., 2007. Surveillance studies on anthelmintic resistance in some gastrointestinal nematodes of Beetal goats at livestock farms of Punjab. Ph.D thesis, Department of Parasitology, University of Agriculture, Faisalabad, Pakistan.
Sangster, N.C., Rickard, J.M., Hennessy, D.R., Steel, J.W. and Collins, G.H., 1991. Disposition of oxfendazole in goats and efficacy compared with sheep. Research in Veterinary Science, 51, 258-263.
Soulsby, E.J.L., 1982. Helminths, Arthropods and Protozoa of Domasticated Animals.7th Ed., Bailliere Tindall, London, UK, pp. 248-250, 766-770.
Taylor, M.A., Coop, R.L. and Wool, R.L., 2007. Veterinary Parasitology, 3rd Ed., Blackwell Publishing Ltd., UK.
Waller, P.J., Dash, K.M., Barger, I.A., Le Jambre, L.F. and Plant, J., 1995. Anthelmintic resistance in nematode parasites of sheep: learning from the Australian experience. Veterinary Record, 136, 411-413.
Published
2020-09-08
How to Cite
Hamad, K. and Qadir, F. (2020) “Rampancy of Antinematicidal Resistance Among Gastrointestinal Nematodes in Kurdish Goat Breeds”, Zanco Journal of Pure and Applied Sciences, 32(4), pp. 98-107. doi: 10.21271/ZJPAS.32.4.12.
Section
Biology and Medical Researches