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homocysteine, vitamin B12, folic acid, Oxidative Stress, lipid profile
An elevated homocysteine (Hcy) level in blood causes endothelial dysfunction and damage. Individuals with higher Hcy concentration have increased risks of cardiovascular disease. Ninety individuals were taken at Hawler Cardiac Surgery Center and diagnosed by coronary angiography. Blood sample collections started in October 2014 and ended in January 2015. The present study includes 60 patients who had stenosis and 30 individuals who had no stenosis as healthy group, patient and healthy groups aged >40 years old. The results of this study show that, the mean ± S.E. levels of tHcy in patient group was 17.170 ± 0.748 μmol/L, which was located above normal range for tHcy and in healthy group was 13.780 ± 0.679 μmol/L, which was located within normal range for Hcy and the concentration of tHcy was significantly higher in patient group compared to healthy group. Also, the mean values of malodialdehyde (MDA) and blood glucose concentrations were significantly higher in patient group than healthy group. Meanwhile, vitamin B12, folic acid levels were significantly higher in healthy group but TAC level was higher and not significant in healthy group when compared to patient group. On the other hand, Tch, TG, LDL and VLDL levels were significantly higher in patient group compared to healthy group. While, HDL level was significantly higher in healthy group rather than patient group. In correlation analysis, tHcy was positively and significantly correlated with LDL (r = 0.338). As well as tHcy was positively (not significantly) correlated with MDA, CH, TG, VLDL and urea, glucose (r = 0.3072; r = 0.07717; r = 0.1063; r = 0.1063; r = 0.2612; r = 0.003459) respectively. While, tHcy was negatively correlated with vitamin B12, folic acid, TAC and HDL (r = -0.02752; r = -0.2076; r = -0.021; r = -0.1554), respectively.
Al-Jumaily, E. F., Khaleel, F. M. and Al-Rawi, A. 2009. The Effect of Chronic liver diseases on homocysteine and vitamin B12 in patients serum. Journal of the Faculty of Medicine. 51 (4), 399-402.
Cavalca, V., Cighetti, G., Bamonti, F., Loaldi, A., Bortone, L., Novembrino, C. andGuazzi, M. D. 2001. Oxidative stress and homocysteine in coronary artery disease. Clinical Chemistry. 47 (5), 887-892.
Chambers, J. C., Ueland, P. M., Obeid, O. A., Wrigley, J., Refsum, H. andKooner, J. S. 2000. Improved vascular endothelial function after oral B vitamins an effect mediated through reduced concentrations of free plasma homocysteine. Circulation. 102 (20), 2479-2483.
Champe, P. C., Harvey, R. A. and Ferrier, D. R. 2008.Lippincott's Illustrated Reviews Biochemistry. 4th Ed. Lippincott Williams and Wilkins, WoltersKlumer. Philadeliphia, USA.
Demirbag, R., Yilmaz, R. andKocyigit, A. 2005. Relationship between DNA damage, total antioxidant capacity and coronary artery disease. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 570 (2), 197-203.
Emoto, M., Kanda, H., Shoji, T., Kawagishi, T., Komatsu, M., Mori, K. andNishizawa, Y. 2001. Impact of insulin resistance and nephropathy on homocysteine in type 2 diabetes. Diabetes Care. 24 (3), 533-538.
Grundy, S. M., Benjamin, I. J., Burke, G. L., Chait, A., Eckel, R. H., Howard, B. V. and Sowers, J. R. 1999. Diabetes and cardiovascular disease a statement for healthcare professionals from the American Heart Association. Circulation. 100 (10), 1134-1146.
Hankey, G. J. andEikelboom, J. W. 1999. Homocysteine and vascular disease. The Lancet. 354 (9176), 407-413.
Kamal, A. H. 2012. Some Haematological Changes in Patients with Cardiovascular Disease.Journal of Babylon University. Pure and Applied Sciences. 20 (2), 538.
Kang, S. S., Wong, P. W. andMalinow, M. R. 1992. Hyperhomocysteinemia as a risk factor for occlusive vascular disease. Annual Review of Nutrition. 12 (1), 279-298.
Kaur, K. Bedi, G., Kaur, M., Vij, A. andKaur, I. 2008. Lipid peroxidation and the levels of antioxidant enzymes in coronary artery disease. Indian Journal of Clinical Biochemistry. 23 (1), 33-37.
Libby, P., Ridker, P. M. andMaseri, A. 2002. Inflammation and atherosclerosis. Circulation. 105 (9), 1135-1143.
McNair, E. D. 2006.Homocysteine and malondialdehyde as predictors of restenosis following percutaneous coronary intervention.M.Sc. Thesis, University of Saskatchewan.
Moat, S. J., Bonham, J. R. and Powers, H. J. 2001. Role of aminothiols as a component of the plasma antioxidant system and relevance to homocysteine-mediated vascular disease. Clinical Science.100 (1), 73-79.
Mohammed, O., Ala’a, G., Hussein, F. andKismat 2011. Study of some Cardiac Biomarkers and Oxidative Stress markers in Patients with Acute coronary syndromes. Karbala Journal Medical. 4(3), 1102-1108.
Navab, M., Hama, S. Y., Cooke, C. J., Anantharamaiah, G. M., Chaddha, M., Jin, L. and Fogelman, A. M. 2000. Normal high density lipoprotein inhibits three steps in the formation of mildly oxidized low density lipoprotein: step 1. Journal of Lipid Research. 41(9), 1481-1494.
Ridker, P. M., Manson, J. E., Buring, J. E., Shih, J., Matias, M. andHennekens, C. H. 1999. Homocysteine and risk of cardiovascular disease among postmenopausal women. Jama. 281(19), 1817-1821.
Sabry, H. H., Sabry, J. H., andHashim, H. M. 2014. Serum levels of homocysteine, vitamin B12, and folic acid in vitiligo. Egyptian Journal of Dermatology and Venerology. 34 (1), 65-69.
Schneeberg, A. 2007.Investigation into the relationship between physical activity and total plasma homocysteine. M.Sc. Thesis, Queen’s University, Canada.
Thakur, S., Gupta, S., Parchwani, H., Shah, V. andYadav, V. 2011. Hs-CRP-A Potential Marker for Coronary Heart Disease. Indian Journal Fundamental Applied Life Science. 1 (1), 1-4.
Toborek, M., Kopieczna-Grzebieniak, E., Drózdz, M. andWieczorek, M. 1995. Increased lipid peroxidation as a mechanism of methionine-induced atherosclerosis in rabbits. Atherosclerosis. 115 (2), 217-224.
Tomás, M., Latorre, G., Sentí, M. andMarrugat, J. 2004. The antioxidant function of high density lipoproteins: a new paradigm in atherosclerosis. Revista Española de Cardiología (English Edition). 57 (6), 557-569.
VanderLaan, P. A., Reardon, C. A. and Getz, G. S. 2004. Site specificity of atherosclerosis site-selective responses to atherosclerotic modulators. Arteriosclerosis, Thrombosis, and Vascular Biology. 24 (1), 12-22.
Warad, S., Kalburgi, N. B., Manak, M., Kalburgi, V. C., Koregol, A. C., Patanashetti, J. andKokatnur, M. V. 2014. Determining the Effect of Gutkha on Serum Levels of Vitamin B12 and Folic Acid as Compared to Smoking among Chronic Periodontitis Subjects: A Cross-Sectional Study. Journal of Clinical and Diagnostic Research for Doctors. 8 (12), 85-89.
Wu, B. T., Dyer, R. A., King, D. J., Richardson, K. J. and Innis, S. M. 2012. Early second trimester maternal plasma choline and betaine are related to measures of early cognitive development in term infants. Plos One. 7 (8), 434- 448.