Detection of Listeria monocytogenes HlyA gene in Food Samples of Meat by Real-Time PCR In Erbil city

Layla Salim, Galawezh Othman

Abstract


The objectives of this study were to evaluate the detection of Listeria monocytogenes in different ready-to-eat foods using real-time PCR (RT-PCR). To monitor L. monocytogenes in foods(meat) Listeria monocytogenes is a gram positive, rod shaped, pathogenic bacterium32 samples (imported frozen breast chicken, 17, local minced beef meat 9 and  imported  minced beef meat 6. were collected from  super  markets in different place in Erbil city . Of all 32samples, 9 samples (28.1%) showed positive signals in RT-PCR. six samples from(imported frozen breast chicken, (35.29%) ,1 samples from  local minced beef meat(11.1%) and two sample from  imported  minced beef meat (33.3%) were determined to be positive for L. monocytogenes .  Also the result showed the lowest CT value at (21.57) The results indicate that the RT-PCR detection method with the set of primers and hybridization probe designed in this study should be useful in monitoring for L. monocytogenes  hlyA gene in meat


Keywords


LISTERIA MONOCYTOGENES, HlyA gene, Real-Time PCR,Meat

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References


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