Fingerprinting and genetic relationship among Escherichia coli strains producing B. Lactamase collected from urine using ERIC/PCR Assay

  • Narmin S. Merza Department of Biology, College of Science, Duhok University, Duhok, Kurdistan Region, Iraq
  • Azzam A. Mosa Department of Chemistry, College of Science, Duhok University, Duhok, Kurdistan Region, Iraq
  • Iman H. Fahd Al-deen Department of Biology, College of Science, Duhok University, Duhok, Kurdistan Region, Iraq
  • Zainab H. Abdullah Department of Biology, College of Science, Duhok University, Duhok, Kurdistan Region, Iraq
  • Zainab H. Abdullah Department of Biology, College of Science, Duhok University, Duhok, Kurdistan Region, Iraq

Abstract





      This study was conducted on 50 isolates recovered from urine samples of patients with urinary infections admitted to Azadi teaching Hospital/Dohuk City. These isolates were identified and characterized in previous study as E. coli producing extended spectrum beta lactamase enzymes, all isolates were subjected to Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC PCR) assay for estimation the fingerprinting and genetic relationship among these isolates. They were successfully producing multiple amplicons ranging from 2-12 bands with different molecular weights ranging from 5212- 213bp. The most frequent band was 724 which was amplified by 31 strains, and the least frequent were 3224, 2424, 1472 and 531bp. observed in only one strain. From the result of dendogram analysis revealed that all isolates were assigned into two main clusters, each cluster was further classified into two subgroups. Similar genotype may indicate clonal spreading of bacteria in hospital wards.



Published
Jun 12, 2018
How to Cite
MERZA, Narmin S. et al. Fingerprinting and genetic relationship among Escherichia coli strains producing B. Lactamase collected from urine using ERIC/PCR Assay. ZANCO Journal of Pure and Applied Sciences, [S.l.], v. 30, n. 3, p. 20-25, june 2018. ISSN 2412-3986. Available at: <http://zancojournals.su.edu.krd/index.php?journal=JPAS&page=article&op=view&path%5B%5D=2061>. Date accessed: 25 june 2018. doi: http://dx.doi.org/10.21271/zjpas.30.3.3.
Section
Articles

Keywords

Fingerprinting, Escherichia coli, Enterobacterial Repetitive Intergenic Consensus, Polymerase Chain Reaction assay, Genetic relationship