Knocking out the pptA gene in Aspergillus fumigatus could result in new protein profile and promising targets for antifungals

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Karzan Mohammad


- Aspergillus fumigatus. - Antifungal target. - Gene knockout. - protein profile.


The filamentous fungus Aspergillus fumigatus is the most considerable common opportunistic pathogen. This is due to the prevalence of its occurrence in patients with immune debilities. It can cause diseases with impact extends to life-threatening seriousness. This fungus recently showed interesting means of adaptation, biofilm formation, and morphogenesis within the host biome. Its ability to invade and systematically disperse in the patient’s body is of great concern. The complications of antifungal therapy and the limited target available to treat such infections, urge intensive and thorough research to uncover new more effective targets. The present study revealed a novel possibility of making the pptA an effective address in the fungus for antifungal treatment. Knocking-out the pptA gene resulted in halting pathogenecity as it correlated with essential genes of pathogenecity medA which showed considerable down regulation. Significant increase of protein profile has been discovered in ∆pptA strains as UBC gene highly activated and protein assay revealed high rate of production. In conclusion, the present study emphasize pptA as a possible strong antifungal target and declares high protein output from ∆pptA which may be a promised strain for industrial and beneficial protein production.

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