Detection of Biofilm Producer Methicillin Resistant Staphylococcus hominis Isolated From Different Clinical Sources

Main Article Content

khadija khalil mustafa

Keywords

Staphylococcus hominis , biofilm, methicillin resistant, mecA

Abstract

Staphylococcus hominis  is an opportunistic gram positive bacteria and is a member of coagulase-negative staphylococci (CoNS) . During the study,  213 clinical specimens were collected from patients admitted to different hospitals in Erbil city, Iraq. Only 18(8.45%)  isolates of S. hominis were isolated  including 7 isolates (17.07%) from blood, 4 isolates (8.70%) from urine, 3 isolates (7.14%) from ear, 2 isolates (6.67%) from wound, and one isolate from each nasal swab (4.55%) and oral cavity (3.13%), all S. hominis identified based on morphology, cultural, biochemical tests, and further confirmed by Vitek 2 compact system. To determine the most accurate assay for measuring methicillin resistance S. hominis, compared the detection of mecA by PCR with detection by National Committee for Clinical Laboratory Standards methods using oxacillin and Cefoxitin disk diffusion method. The results of oxacillin showed 13 (72.22%) isolates resistant to methicillin and 5 (27.77%) isolates were sensitive to it. While, the results of cefoxitin demonstrated that 16 (88.89%) isolates were resistant to methicillin and only 2 isolates (11.11%) were sensitive to it.  However, the same results of the Cefoxitin disk diffusion method was obtained by  PCR and by using  mecA gene which 16 isolates (88.89%) were carried mecA gene with product size 499bp. The results of microtiter plate method revealed that 16 (88.89%)  isolates of  S. hominis were  biofilm  producer  and  only 2 isolates (11.11%) were  non-biofilm producer. Moreover,of  16 biofilm producer isolates, 14 isolates (77.77%) were categorized as strong biofilm producers and 2 (11.11%) isolates were  identified as moderate biofilm producers

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References
1. ABDULLAH, ZH. & BARZANI, KH. KH. 2016. Bacteriological and Molecular Study of Gram Positive Bacteria Isolated From Thalassemic Patients in Erbil City. Zanco Journal of Pure and Applied Sciences. 28.
2. ABDULLAH, ZH. & BARZANI, KH. KH. 2017. Characterization and Detection of mecA gene in Different Species of Staphylococcus, Streptococcus and Kocuria Which Isolated from Thalassemia Patients in Erbil City. Cihan University Erbil Scientific Journal, 2, DOI: http://dx.doi.org/10.24086/cuesj.si.2017.n2a8.
3. ATLAS, R. M. 2010. Handbook of microbiological media. 4th Education. Library of Congress Cataloging-in-Publication Data. New York. USA.
4. AL WOHOUSH, I.; RIVERA, J., CAIRO, J. ; HACHEM, R. & RAAD, I. 2010. Comparing clinical and microbiological methods for the diagnosis of true bacteraemia among patients with multiple blood cultures positive for coagulase negative staphylococci. Clin Microbiol Infect, 17(4), 569–71. doi:10.1111/j.1469-0691.2010. 03372.xPMID:20854425.
5. BANNERMAN, T and PEACOCK, SJ .2007. Staphylococcus, Micrococcus, andother catalase-positive cocci. In: Murray PR, Baron EJ, Jorgensen JH et al., eds. Manual of Clinical Microbiology. Washington: ASM Press,390–411.
6. BARZANI, KH. KH.; ABDULLAH, ZH.; HYDAEAT, S. & MOOHAMAD, A. 2016. Detection of Tetracycline Resistant Gen (tet K, tet M ) in Some Coagulase Negative Staphylococci Isolated From Different Clinical Sources In Erbil City. J. of University of Anbar for pure science : Vol.10:NO.1; ISSN: 1991-8941.
7. BHAT, R.; DESHPANDE, R.; GANACHARI, SH.; HUH, D. & VENKATARAMAN, A. 2011. Photo-Irradiated Biosynthesis of Silver Nanoparticles Using Edible Mushroom Pleurotus florida and Their Antibacterial Activity Studies. Hindawi Publishing Corporation Bioinorganic Chemistry and Applications. Article ID 650979, 7p. doi:10.1155/2011/650979.
8. BOUCHAMI, O.; BEN, H. A.; DE LENCASTRE, H. & MIRAGAIA, M.. 2011. Molecular epidemiology of methicillin-resistant Staphylococcus hominis (MRSHo): low clonality and reservoirs of SCC mec structural elements. PLoS One. 6(7):e21940.
9. BRASEL, K. J. & Weigelt, J. A. 2007. Community-Acquired MRSA as a Pathogen.P.43-54. In, J.A. Weigelt (ed.). MRSA. Informa Healthcare, New York.
10. CALDERON-JAIMES, E.; Monteros, L.E. & Avila-Beltrán, R. 2002. Epidemiology of drug resistance: The case of Staphylococcus aureus and coagulase-negative staphylococci infections. salud pública de méxico.44(2):108-112.
11. CHAMBERS, H. F. 1988. Methicillin-Resistant Staphylococci. Clin. Microbiol. Rev. 1(2):173-186.
12. CHAVES, F.; GARCIA-ALVAREZ, M.; SANZ, F.; ALBA, C. & OTERO, J. 2005. Nosocomial Spread of a Staphylococcus hominis subsp. novobiosepticus Strain Causing Sepsis in a Neonatal Intensive Care Unit. J. Clin. Microbiol, (43) 9; 4877 -4879. doi: 10.1128/JCM.43.9.4877-4879.2005 .
13. CHEESBROUGH, M. 2006. District labrotary practice in tropical countries. Tropical Health Technology, 2: 51.
14. CHRISTENSEN, G.D.; SIMPSON, W. A.; YONGER, J. J.; BADDOR, L. M.; BARRETT, F. F.; MELTON, D. M. & BEACHEY, E. H. 1985. Adherence of coagulase-negative Staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 22:996-1006.
15. CLINICAL, AND LABORATORY STANDARDS INSTITUTE (CLSI) 2015. Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Five Informational Supplement M100-S25. Wayne, PA, USA,.
16. DAVIES D. 2003. Understanding biofilm resistance to antibacterial agents. Nat Rev Drug Discov., 2(2),114–22. PMID:12563302.
17. D'AZEVEDO, P.A.; TRANCESI, R.; SALES, T.; MONTEIRO, J.; GALES, A. C. & PIGNATAR, A. C. 2008. Outbreak of Staphylococcus hominis subsp.novobiosepticus bloodstream infections in Sao Paulo city, Brazil. J Med Microbiol., 57(Pt 2),256–7. doi:10.1099/jmm.0.47345-0PMID:1820199
18. ENRIGHT, M. C.; ROBINSON, D. A.; RANDLE, G.; FEIL, E. J.; GRUNDMANN, H. & SPRATT, B. G. 2002. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). PNAS. 99(11):7687-7692.
19. FREDHEIM, E. G; KLINGENBERG, C.; ROHDE, H.; FRANKENBERGER, S.; GAUSTAD, P.; FLAEGSTAD, T. et al. 2009. Biofilm formation by Staphylococcus haemolyticus. J Clin Microbiol. 47(4),1172–80. doi:10.1128/JCM.01891-08PMID:19144798.
20. FUNG-TOMC, J. C.; CLARK, J.; MINASSIAN, B.; PUCCI, M.; TSAI, Y.; GRADELSKI, E.; LAMB, L.; MEDINA, I.; HUCZKO, E.; KOLEK, B.; CHANIEWSKI, S.; FERRARO, C. l.; WASHO, T. & BONNER, D. P. 2002. In Vitro and In Vivo Activities of a Novel Cephalosporin, BMS-247243, against Methicillin Resistant and Susceptible Staphylococci. Antimicrob. Agents Chemother. 46(4):971-976.
21. GARZA-GONZALEZ, E.; MORFIN-OTERO, R.; MARTINEZ-VAZQUEZ, M. A.; GONZALEZ-DIAZ, E.; GONZALEZ-SANTIAGO, O. & RODRIGUEZ-NORIEGA, E. 2011. Microbiological and molecular characterization of human clinical isolates of Staphylococcus cohnii, Staphylococcus hominis, and Staphylococcus sciuri. Scand J Infect Dis.; 43(11-12): 930-6.
22. GOTZ, F. 2006. The genera Staphylococcus and Macrococcus, p. 5–75. In: Dworkin M., S. Falkow, E. Rosenberig, K-H. Schleiferr, E. Stackebrands (eds). The Prokaryotes, 3nd A Handbook on the biology of bacteria: Firmicutes, Cyanobacteria.
23. HARLEY, J. P. & PRESCOTT, L. M. 2002. Laboratory Exercises in Microbiology. 5th .ed. The McGraw-Hill Companies, Inc., New York. USA.
24. HOIBY, N.; BJARNSHOLT, T.; GIVSKOY, M.; MOLIN, S. & CIOFU, O. 2010. Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents.,35(4), 322–32. doi:10.1016/j.ijantimicag. 2009.12.011PMID:20149602.
25. JABRA-RIZK, M. A.; MEILLER, T. F.; JAMES, C. E. & SHIRTLIFF, M. E. 2006. Effect of Farnesol on Staphylococcus aureus Biofilm Formation and Antimicrobial Susceptibility. Antimicrob. Agents Chemother, 50(4). p1463-1469.
26. KHAN, S.; SHETTY, P. J.; SARAYU, L. Y.; CHIDAMBARAM, A. & RANGANATHAN, R. 2012. Detection of mecA genes of Methicillin-Resistant Staphylococcus aureus by Polymerase Chain Reaction. International Journal of Health and Rehabilitation Sciences. 1(2).p. 64-68.
27. KLOOS, W. E. & SCHLEIFER, K. H. 1986. Genus IV. Staphylococcus, p. 1013 – 1035. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt. (ed.), Bergey’s manual of systematic bacteriology, vol. 2.Williams & Wilkins, Baltimore, MD.
28. MATHUR, T.; SINGHAL, S.; KHAN, S.; UPADHYAY, D. J.; FATMA, T. & RATTAN, A. 2006. Detection of Biofilm Formation among the Clinical Isolates of Staphylococci: An Evaluation of Three Different Screening Methods. Indian J. Med. Microbiol. 24(1). p 25-29.
29. MENDOZA-OLAZARAN S.; MORFIN-OTERO, R.; RODRIGUEZ-NORIEGA, E.; LLACA-DIAZ, J.; FLORES-TREVINO, S.; GONZALEZ-GONZALEZ, G.M. & et al. 2013. Microbiological and molecular characterization of Staphylococcus hominis isolates from blood. PLoS One., 8(4). p 61161.
30. MENDOZA-OLAZARAN, S.; MORFIN-OTERO, R.; VILLARREAL-TREVINO, L.; RODRIGUEZ-NORIEGA, E.; LLACA-DIAZ, J.; CAMACHO-ORTIZ, A.; GONZALEZ, G.; CASILLAS-VEGA, N. & GARZA-GONZALEZ, E. 2015. Antibiotic Susceptibility of Biofilm Cells and Molecular Characterization of Staphylococcus hominis Isolates from Blood. PLOS ONE | DOI: 10.1371/journal. pone.0144684.
31. NOVICK, R. P. & GEISINGER, E. 2008. Quorum sensing in staphylococci. Annu Rev Genet, 42. p 541-564.
32. PALAZZO, I. C.; AZEVEDO, P. A.; SECCHI, C.; PIGNATARI, A. & DARINI, A. L. 2008. Staphylococcus hominis subsp. Novobio septicus strains causing nosocomial bloodstream infection in Brazil. Journal of Antimicrobial Chemotherapy. 62. p 1222–1226. doi:10.1093/jac/dkn375.
33. RODHE, H.; MACK, D.; CHRISTNER, M.; BURDESKI, C.; FRANKE, G. & KNOBLOCH, J. K. M. 2006. Pathogenesis of staphylococcal devicerelated infections: from basic science to new diagnostic, therapeutic and prophylactic approaches. Rev. Med. Microbiol.17: 45–54.
34. SOROUSH, S.; ABALAMELI, J. F.; TAHERIKALANI, M.; ESLAMPOUR, M.; BEIGVERDI, R. & EMANEINI, M. 2017. Characterization of biofilm formation, antimicrobial resistance, and staphylococcal cassette chromosome mecanalysis of methicillin resistant Staphylococcus hominis from blood cultures of children. Rev Soc Bras Med Trop 50(3). p329-333, doi: 10.1590/0037-8682-0384.
35. SZCZUKA, E.; TRAWCZYŃSKI, K. & KAZNOWSKI A. 2014. Clonal Analysis of Staphylococcus hominis Strains Isolated from Hospitalized Patients. Journal of Microbiology. 63 (3). p 349–354.
36. ZHANG, L.; THOMAS, J. C.; MIRAGAIA, M.; BOUCHAMI, O.; CHAVES, F.; d'AZEVEDO, P. A. & et al. 2013. Multilocus sequence typing and further genetic characterization of the enigmatic pathogen, Staphylococcus hominis. PLoS One., 8(6). p66496.