Cloning, Expression and Purification of Trypsin-like Protease of the Periodontal Pathogen Tannerella forsythia ATCC 43037
Tannerella forsythia, a bacterial species frequently associated with the pathogenesis of periodontal disease and its proteases are implicated as virulence factors, is known to possess trypsin-like activity. Here, we characterize a trypsin-like protease of T. forsythia referred to as tlp. Full length (without a signal peptide) recombinant tlp (102 kDa). The evidence suggests that the enzyme is a serine protease since it was strongly inhibited by Pefabloc, Tosyl-L-Lysyl-chloromethane hydrochloride (TLCK) and Leupeptin. The ability to degrade elastin, fibrinogen and the antimicrobial peptide LL-37 may contribute to the pathogenicity of periodontitis.
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